Human/Mouse/Rat Casein Kinase 1 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4569
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CK1 alpha
Ala255-Phe365
Accession # NP_001020276
Ala255-Phe365
Accession # NP_001020276
Specificity
Detects endogenous human, mouse, and rat CK1 alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CK1 delta or CK1 gamma; reactivity with CK1 epsilon is unknown.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Casein Kinase 1 alpha Antibody
Detection of Human and Rat Casein Kinase 1 alpha by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Casein Kinase 1a at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Casein Kinase 1 alpha Specificity Using Knockdown Cell Line.
Western blot shows lysates of HCT 116 human colorectal carcinoma cell line and Casein Kinase 1 alpha knockdown HCT 116 cell line (KD). Nitrocellulose membrane was probed with 2 µg/mL of Human/Mouse/Rat Casein Kinase 1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) followed by HRP-conjugated donkey anti-sheep IgG Secondary Antibody. A specific band was detected for Casein Kinase 1 alpha at approximately 36 kDa (as indicated) in the parental HCT 116 cell line, but is significantly reduced in knockdown HCT 116 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of Casein Kinase 1 alpha by Immunoprecipitation.
HCT 116 lysates were prepared and immunoprecipitation was performed using 2.0 µg of Human/Mouse/Rat Casein Kinase 1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) pre-coupled to Dynabeads protein G. Immunoprecipitated Casein Kinase 1 alpha was detected with a Rabbit Anti-Casein Kinase 1 alpha antibody. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).Applications for Human/Mouse/Rat Casein Kinase 1 alpha Antibody
Application
Recommended Usage
Immunocytochemistry
2 µg/mL
Sample: HCT 116 human colorectal carcinoma cell line
Sample: HCT 116 human colorectal carcinoma cell line
Immunoprecipitation
2 µg/1 mg cell lysate
Sample: Lysates of HCT 116 human colorectal carcinoma cell line
Sample: Lysates of HCT 116 human colorectal carcinoma cell line
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line,K562 human chronic myelogenous leukemia cell line, Y3-Ag rat myeloid cell line, and HCT 116 human colorectal carcinoma cell line
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line,K562 human chronic myelogenous leukemia cell line, Y3-Ag rat myeloid cell line, and HCT 116 human colorectal carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 1 alpha
Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma1, gamma2, gamma3, delta, and epsilon) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 alpha is speculated to play a role in mammalian cell cycle progression, spindle dynamics, and chromosome segregation.
Alternate Names
CSNK1A1, HLCDGP1
Gene Symbol
CSNK1A1
UniProt
Additional Casein Kinase 1 alpha Products
Product Documents for Human/Mouse/Rat Casein Kinase 1 alpha Antibody
Product Specific Notices for Human/Mouse/Rat Casein Kinase 1 alpha Antibody
For research use only
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