Human/Mouse/Rat CD2AP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4474
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CD2AP
Ile423-Lys580
Accession # Q9Y5K6
Ile423-Lys580
Accession # Q9Y5K6
Specificity
Detects endogenous human, mouse, and rat CD2AP in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat CD2AP Antibody
Detection of Human/Mouse/Rat CD2AP by Western Blot.
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, KG-1 human acute myelogenous leukemia cell line, M1 mouse myeloid leukemia cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat CD2AP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat CD2AP Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, Y3-Ag rat myeloid cell line, KG-1 human acute myelogenous leukemia cell line, and M1 mouse myeloid leukemia cell line
Sample: MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, Y3-Ag rat myeloid cell line, KG-1 human acute myelogenous leukemia cell line, and M1 mouse myeloid leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD2AP
CD2AP (CD2-associated protein; also known as CMS) is an 80 kDa member of the CIN85/CD2AP family of adaptor proteins. Although CD2AP is ubiquitously expressed, it is often associated with glomerular podocytes. CD2AP serves as a scaffold between membrane proteins and the cytoskeleton. Through a Pro-rich region and SH3 domains, it is known to bind to such proteins as CD2, c-CBL, and p130Cas. Human CD2AP is 639 amino acids (aa) in length. It contains three SH3 homology domains (aa 4‑324), one Pro-rich region (aa 336‑422) and a C-terminal coiled-coil region that mediates homodimerization. Over aa 423‑580, human CD2AP shares 96% and 79% aa identity with dog and mouse CD2AP, respectively.
Long Name
CD2-associated protein
Alternate Names
CMS
Gene Symbol
CD2AP
UniProt
Additional CD2AP Products
Product Documents for Human/Mouse/Rat CD2AP Antibody
Product Specific Notices for Human/Mouse/Rat CD2AP Antibody
For research use only
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