Human/Mouse/Rat CDK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4654
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Xenograft
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CDK2
Met1-Leu298
Accession # P24941
Met1-Leu298
Accession # P24941
Specificity
Detects human, mouse, and rat CDK2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) CDK4 and rhCDK6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat CDK2 Antibody
Detection of Human/Mouse CDK2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). For additional reference, recombinant human CDK2, CDK4, and CDK6 (5 ng/lane) were included. A specific band for CDK2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat CDK2 by Western Blot.
Western blot shows lysates of C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CDK2 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CDK2 in K562 Human Cell Line.
CDK2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Applications for Human/Mouse/Rat CDK2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line and immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line and immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Simple Western
20 µg/mL
Sample: Lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line.
Sample: Lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line.
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF4654 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDK2
Long Name
Cyclin-dependent Kinase 2
Alternate Names
cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)
Gene Symbol
CDK2
UniProt
Additional CDK2 Products
Product Documents for Human/Mouse/Rat CDK2 Antibody
Product Specific Notices for Human/Mouse/Rat CDK2 Antibody
For research use only
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