Human/Mouse/Rat Chk1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1630
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Chk1
Tyr157-Thr476
Accession # O14757
Tyr157-Thr476
Accession # O14757
Specificity
Detects human, mouse, and rat Chk1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Chk1 Antibody
Detection of Human, Mouse, and Rat Chk1 by Western Blot.
Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, CEM human T-lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Chk1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Chk1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Chk1 Antibody
Application
Recommended Usage
Immunoprecipitation
2 µg/500 µg cell lysate
Sample: U2OS human osteosarcoma cell line and Balb/3T3 mouse embryonic fibroblast cell line , see our available Western blot detection antibodies
Sample: U2OS human osteosarcoma cell line and Balb/3T3 mouse embryonic fibroblast cell line , see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, CEM human T-lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, CEM human T-lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk1
Long Name
Checkpoint Kinase 1
Alternate Names
CHEK1
Gene Symbol
CHEK1
UniProt
Additional Chk1 Products
Product Documents for Human/Mouse/Rat Chk1 Antibody
Product Specific Notices for Human/Mouse/Rat Chk1 Antibody
For research use only
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