Human/Mouse/Rat Creatine Kinase BB Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9076

R&D Systems, part of Bio-Techne
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MAB9076-100
MAB9076-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 969409

View all Creatine Kinase BB Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Creatine Kinase BB
Pro2-Lys381
Accession # P12277

Specificity

Detects human Creatine Kinase BB in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Creatine Kinase BB Antibody

Detection of Human, Mouse, and Rat Creatine Kinase BB antibody by Western Blot.

Detection of Human, Mouse, and Rat Creatine Kinase BB by Western Blot.

Western blot shows lysates of mouse and rat brain (cerebellum) tissue, human brain (motor cortex and hippocampus) tissue, and human prostate tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/ Mouse/Rat Creatine Kinase BB Monoclonal Antibody (Catalog # MAB9076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Creatine Kinase BB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Creatine Kinase BB antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

Creatine Kinase BB in SH‑SY5Y Human Cell Line.

Creatine Kinase BB was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat Creatine Kinase BB Monoclonal Antibody (Catalog # MAB9076) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Creatine Kinase BB Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line

Western Blot

0.1 µg/mL
Sample: Human brain (motor cortex and hippocampus) tissue, human prostate tissue, and mouse and rat brain (cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Creatine Kinase BB

Creatine kinase (CK) catalyzes the conversion of creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP). CK reaction is reversible and thus ATP can be generated from PCr and ADP (1). In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit (2). Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure. Creatine kinase B (CKB), can form homodimer (BB type), and heterodimer (MB type) with creatine kinase M (CKM). MB type is found in myocardium, and homodimer BB type is found in many tissues, especially brain. The recombinant human CKB is in the BB type and its activity is measured using a phosphatase-coupled method (4).

References

  1. Trask R.V. et al. (1988) J. Biol. Chem. 263:17142.
  2. Wallimann, T. et al. (1992) Biochem, J. 281:21-40.
  3. Wallimann, T. (1994) Curr. Biol. 4:42.
  4. Wu, Z.L. (2011) PLoS One 6:e23172.

Alternate Names

B-CK, CKB

Entrez Gene IDs

1152 (Human); 102121556 (Cynomolgus Monkey)

Gene Symbol

CKB

UniProt

P12277

Additional Creatine Kinase BB Products

Product Documents for Human/Mouse/Rat Creatine Kinase BB Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Creatine Kinase BB Antibody

For research use only

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