Human/Mouse/Rat CUGBP1/CELF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9388
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 850717
Product Specifications
Immunogen
E. coli-derived recombinant human CUGBP1/CELF1
Met1-Gly60
Accession # Q92879
Met1-Gly60
Accession # Q92879
Specificity
Detects human CUGBP1/CELF1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat CUGBP1/CELF1 Antibody
Detection of Human, Mouse, and Rat CUGBP1/CELF1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat CUGBP1/ CELF1 Monoclonal Antibody (Catalog # MAB9388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CUGBP1/CELF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CUGBP1/CELF1 in Human Skeletal Muscle.
CUGBP1/CELF1 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat CUGBP1/CELF1 Monoclonal Antibody (Catalog # MAB9388) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat CUGBP1/CELF1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CUGBP1/CELF1
Long Name
CUGBP, Elav-Like Family Member 1
Alternate Names
BRUNOL2, CELF1, CUG-BP, CUG-BP1, D2Wsu101e, EDEN-BP, HNAB50, NAB50, NAPOR
Gene Symbol
CELF1
UniProt
Additional CUGBP1/CELF1 Products
Product Documents for Human/Mouse/Rat CUGBP1/CELF1 Antibody
Product Specific Notices for Human/Mouse/Rat CUGBP1/CELF1 Antibody
For research use only
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