Human/Mouse/Rat DARPP-32 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6259
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DARPP‑32
Arg51-Ala204
Accession # Q9UD71
Arg51-Ala204
Accession # Q9UD71
Specificity
Detects human, mouse, and rat DARPP‑32 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat DARPP-32 Antibody
Detection of Human, Mouse, and Rat DARPP‑32 by Western Blot.
Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DARPP-32 at approximately 32 - 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DARPP‑32 in Human Brain.
DARPP-32 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
DARPP‑32 in Mouse Brain.
DARPP-32 was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse/Rat DARPP-32 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus), and perfusion fixed frozen sections of mouse brain (caudate putamen) and rat brain (globus pallidus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus), and perfusion fixed frozen sections of mouse brain (caudate putamen) and rat brain (globus pallidus)
Western Blot
1 µg/mL
Sample: Human brain tissue, mouse brain tissue, and rat brain tissue
Sample: Human brain tissue, mouse brain tissue, and rat brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DARPP-32
Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kDa protein that anomalously migrates at about 32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP‑32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.
Long Name
Dopamine and cAMP-regulated Phosphoprotein
Alternate Names
DARPP32, PPP1R1B
Gene Symbol
PPP1R1B
UniProt
Additional DARPP-32 Products
Product Documents for Human/Mouse/Rat DARPP-32 Antibody
Product Specific Notices for Human/Mouse/Rat DARPP-32 Antibody
For research use only
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