Human/Mouse/Rat Dihydrofolate Reductase/DHFR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7934
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 872442
Product Specifications
Immunogen
E. coli-derived recombinant human Dihydrofolate Reductase/DHFR
Met1-Asp187
Accession # P00374
Met1-Asp187
Accession # P00374
Specificity
Detects human Dihydrofolate Reductase/DHFR in ELISA. Detects human, mouse and rat Dihydrofolate Reductase in Western Blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Dihydrofolate Reductase/DHFR Antibody
Detection of Human, Mouse, and Rat Dihydrofolate Reductase/DHFR by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dihydrofolate Reductase/DHFR at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Dihydrofolate Reductase/DHFR in MCF‑7 Human Cell Line.
Dihydrofolate Reductase/DHFR was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Dihydrofolate Reductase/DHFR in C2C12 Mouse Cell Line.
Dihydrofolate Reductase/DHFR was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Dihydrofolate Reductase/DHFR Monoclonal Antibody (Catalog # MAB7934) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Dihydrofolate Reductase/DHFR Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line and MCF‑7 human breast cancer cell line
Sample: Immersion fixed C2C12 mouse myoblast cell line and MCF‑7 human breast cancer cell line
Immunohistochemistry
8-25 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human colon
Sample: Formalin fixed paraffin-embedded sections of human colon
Simple Western
10 µg/mL
Sample: 293T human embryonic kidney cell line
Sample: 293T human embryonic kidney cell line
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, Rat‑2 rat embryonic fibroblast cell line
Sample: 293T human embryonic kidney cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dihydrofolate Reductase/DHFR
Alternate Names
DHFR, DHFRP1, DYR
Gene Symbol
DHFR
UniProt
Additional Dihydrofolate Reductase/DHFR Products
Product Documents for Human/Mouse/Rat Dihydrofolate Reductase/DHFR Antibody
Product Specific Notices for Human/Mouse/Rat Dihydrofolate Reductase/DHFR Antibody
For research use only
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