Human/Mouse/Rat DOK7 Antibody

Detection of Human, Mouse, and Rat DOK7 by Western Blot.

Western blot shows lysates of untreated MCF-7 human breast cancer cell line and T47D human breast cancer cell line and C2C12 mouse myoblast cell line and L6 rat myoblast cell line untreated (-) or treated (+) with 2% horse serum for 6 days. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOK7 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human DOK7 by Simple Western^TM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for DOK7 at approximately 60 & 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse DOK7 by Western Blot

Sorbs1 is enriched at AChR aggregates, and Sorbs1 RNAi blocks AChR clustering in vitro. (A) Treatment of myotubes with siRNA directed against Sorbs1 blocks AChR clustering in C2C12 cells. Montages containing sixteen fields at a magnification of 10× were analyzed with ImageJ software (NIH). (B) Sorbs1 siRNA significantly reduces Sorbs1 protein expression in myotubes. (C) Sorbs1 protein is highly enriched at sites where AChRs aggregate. (D) Agrin stimulates tyrosine phosphorylation of MuSK and Dok-7 at similar levels in myotubes treated with Sorbs1 siRNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26527617), licensed under a CC-BY license. Not internally tested by R&D Systems.