Human/Mouse/Rat DYRK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5409
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DYRK3
Asn52-Glu167
Accession # O43781
Asn52-Glu167
Accession # O43781
Specificity
Detects endogenous human, mouse and rat DYRK3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat DYRK3 Antibody
Detection of Human/Mouse/Rat DYRK3 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat DYRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5409) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DYRK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat DYRK3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DYRK3
DYRK3 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 3; also REDK) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK3 is expressed in testis, erythroid-lineage precursors and other tissues, and shows dual substrate specificity. Autophosphorylation on Tyr369 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include CREB and histone 2B. Human DYRK3 is 588 amino acids (aa) in length, and contains one kinase catalytic domain (aa 209‑522). There are two potential isoform variants that show either a six aa substitution for aa 1‑26, or this same substitution in combination with a premature truncation after Gln263.
Long Name
Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 3
Alternate Names
DYRK5, REDK, YAK3-2
Gene Symbol
DYRK3
UniProt
Additional DYRK3 Products
Product Documents for Human/Mouse/Rat DYRK3 Antibody
Product Specific Notices for Human/Mouse/Rat DYRK3 Antibody
For research use only
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