Human/Mouse/Rat ERK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF12301
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ERK2
Met1-Ser360
Accession # P28482
Met1-Ser360
Accession # P28482
Specificity
Detects human, mouse, and rat ERK2. Also recognizes ERK1 in some cell types at much lower affinity.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat ERK2 Antibody
Detection of Human and Mouse ERK2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, TS1 mouse helper T cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF12301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ERK2 in Human Breast.
ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF12301) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Western Blot Shows Human ERK2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF12301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat ERK2 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13-15), perfusion fixed frozen sections of rat brain, and immersion fixed paraffin-embedded sections of human breast
Sample: Immersion fixed frozen sections of mouse embryo (E13-15), perfusion fixed frozen sections of rat brain, and immersion fixed paraffin-embedded sections of human breast
Knockout Validated
ERK2
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
ERK2 knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, TS1 mouse helper T cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, TS1 mouse helper T cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK2
ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.
ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.
Long Name
Extracellular Signal-regulated Kinase 2
Alternate Names
ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2
Gene Symbol
MAPK1
UniProt
Additional ERK2 Products
Product Documents for Human/Mouse/Rat ERK2 Antibody
Product Specific Notices for Human/Mouse/Rat ERK2 Antibody
For research use only
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