Human/Mouse/Rat FABP1/L-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2964
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 328607
Product Specifications
Immunogen
E. coli-derived recombinant human FABP1/L‑FABP
Met1-Ile127
Accession # AAA52418
Met1-Ile127
Accession # AAA52418
Specificity
Detects human FABP1/L‑FABP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FABP2, -3, -4, -5, -6, -7, or recombinant mouse FABP9 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat FABP1/L-FABP Antibody
Detection of Human FABP1/L-FABP by Western Blot.
Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB2964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/ L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/ L-FABP Monoclonal Antibody (Catalog # MAB2964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP1/L‑FABP in Human Kidney.
FABP1/L-FABP was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB2964) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat FABP1/L-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Simple Western
5 µg/mL
Sample: Mouse liver tissue
Sample: Mouse liver tissue
Western Blot
0.25 µg/mL
Sample: Human liver tissue, human colon tissue, human kidney tissue, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue
Sample: Human liver tissue, human colon tissue, human kidney tissue, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue
Reviewed Applications
Read 4 reviews rated 4 using MAB2964 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP1/L-FABP
FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.
Long Name
Fatty Acid-Binding Protein 1
Alternate Names
L-FABP, LFABP
Gene Symbol
FABP1
UniProt
Additional FABP1/L-FABP Products
Product Documents for Human/Mouse/Rat FABP1/L-FABP Antibody
Product Specific Notices for Human/Mouse/Rat FABP1/L-FABP Antibody
For research use only
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