Human/Mouse/Rat FABP2/I-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB30782
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 989821
Product Specifications
Immunogen
E. coli-derived recombinant human FABP2/I-FABP
Met1-Asp132
Accession # P12104
Met1-Asp132
Accession # P12104
Specificity
Detects human FABP2/I-FABP in direct ELISAs. Detects human, mouse, and rat FABP2/I-FABP in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat FABP2/I-FABP Antibody
Detection of Human, Mouse, and Rat FABP2/I-FABP by Western Blot.
Western blot shows lysates of human small intestine tissue, mouse small intestine tissue, and rat small intestine tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat FABP2/I-FABP Monoclonal Antibody (Catalog # MAB30782) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP2/I-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP2/I‑FABP in Human Small Intestine.
FABP2/I-FABP was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human/Mouse/Rat FABP2/I-FABP Monoclonal Antibody (Catalog # MAB30782) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat FABP2/I-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine
Sample: Immersion fixed paraffin-embedded sections of human small intestine
Western Blot
0.5 µg/mL
Sample: Human small intestine tissue, Mouse small intestine tissue, and Rat small intestine tissue
Sample: Human small intestine tissue, Mouse small intestine tissue, and Rat small intestine tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP2/I-FABP
Intestinal Fatty Acid Binding Protein (FABP2), also known as I-FABP or gFABP is a member of the fatty acid binding protein family of intracellular proteins. FABP2 is only expressed in intestine, and it mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variation of FABP2 is implicated in obesity and Type II diabetes.
Long Name
Fatty Acid-Binding Protein 2/Intestinal FABP
Alternate Names
I-FABP, IFABP, Intestinal FABP
Gene Symbol
FABP2
UniProt
Additional FABP2/I-FABP Products
Product Documents for Human/Mouse/Rat FABP2/I-FABP Antibody
Product Specific Notices for Human/Mouse/Rat FABP2/I-FABP Antibody
For research use only
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