Human/Mouse/Rat FLIP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8430
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 896537
Product Specifications
Immunogen
E. coli-derived recombinant human FLIP
Met1-Asn200
Accession # O15519
Met1-Asn200
Accession # O15519
Specificity
Detects human FLIP in direct ELISAs and human, mouse, and rat FLIP in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat FLIP Antibody
Detection of Human, Mouse, and Rat FLIP by Western Blot.
Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line, L-929 mouse fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FLIP at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FLIP in Human Pancreatic Cancer Tissue.
FLIP was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cells in islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat FLIP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Western Blot
2 µg/mL
Sample: PANC‑1 human pancreatic carcinoma cell line, L‑929 mouse fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: PANC‑1 human pancreatic carcinoma cell line, L‑929 mouse fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FLIP
Long Name
FLICE Inhibitory Proteins
Alternate Names
CASPER, CFLAR, FLAME-1, I-FLICE
Gene Symbol
CFLAR
UniProt
Additional FLIP Products
Product Documents for Human/Mouse/Rat FLIP Antibody
Product Specific Notices for Human/Mouse/Rat FLIP Antibody
For research use only
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