Human/Mouse/Rat GRP75/HSPA9B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3584
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GRP75
Glu542-Gln679
Accession # P38646
Glu542-Gln679
Accession # P38646
Specificity
Detects human, mouse, and rat GRP75.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat GRP75/HSPA9B Antibody
Detection of Human/Mouse/Rat GRP75/HSPA9B by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRP75/HSPA9B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
GRP75/HSPA9B in HeLa Human Cell Line.
GRP75/HSPA9B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3584) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GRP75/HSPA9B in Human Meningioma.
GRP75/HSPA9B was detected in immersion fixed paraffin-embedded sections of human meningioma using 15 µg/mL Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3584) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat GRP75/HSPA9B Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human meningioma and colon cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human meningioma and colon cancer tissue
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRP75/HSPA9B
The 70 kDa heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and constitutively expressed members known as heat shock cognate proteins. Glucose Regulated 75 kDa Protein (GRP75, also known as HSPA9B, mitochondrial HSP70, and mortalin-2) is a 679 amino acid (aa) heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. GRP75 is a mitochondrial protein involved in protein translocation into the mitochondria. Proteins crossing the mitochondrial membrane require unfolding before entering translocation pores in the mitochondrial outer membrane. GRP75 together with other inner membrane proteins of the mitochondria mediate this process. GRP75 also plays a role in the control of cell cycle progression.
Long Name
75 KD Glucose Regulated Protein
Alternate Names
CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74
Gene Symbol
HSPA9
UniProt
Additional GRP75/HSPA9B Products
Product Documents for Human/Mouse/Rat GRP75/HSPA9B Antibody
Product Specific Notices for Human/Mouse/Rat GRP75/HSPA9B Antibody
For research use only
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