Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7679
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Histone Deacetylase 2/HDAC2
Pro386-Pro488
Accession # Q92769
Pro386-Pro488
Accession # Q92769
Specificity
Detects human, mouse, and rat Histone Deacetylase 2/HDAC2 in Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human HDAC1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody
Detection of Human, Mouse, and Rat Histone Deacetylase 2/HDAC2 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Rat-2 rat embryonic fibroblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7679) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Rat‑2 rat embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: K562 human chronic myelogenous leukemia cell line, Rat‑2 rat embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone Deacetylase 2/HDAC2
HDAC2 (Histone deacetylase 2; also HD2) is a 55-60 kDa nuclear member of the class I subfamily, histone deacetylase family of transcriptional regulators. It is found in a variety of cell types including monocytes, cardiac muscle cells and neurons. HDAC2 is also crucial for embryonic development. HDAC2 catalyzes the hydrolytic release of acetyl groups from acetylated histone proteins. Within the context of a large complex, this has the effect of repressing gene activity. Human HDAC2 is 488 amino acids (aa) in length. It contains one histone deacetylation domain (aa 29-388), plus sites for phosphorylation, nitrosylation, ubiquitination, and nitration. There is one alternative start site at Met31. Over aa 386-488, human HDAC2 shares 98% and 53% aa sequence identity with mouse HDAC2 and human HDAC1, respectively.
Long Name
Histone Deacetylase 2
Alternate Names
HDAC2, YAF1
Gene Symbol
HDAC2
UniProt
Additional Histone Deacetylase 2/HDAC2 Products
Product Documents for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody
Product Specific Notices for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody
For research use only
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