Human/Mouse/Rat JAB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6390
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human JAB1
aa 158-334
Accession # Q92905
aa 158-334
Accession # Q92905
Specificity
Detects human, mouse, and rat JAB1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat JAB1 Antibody
Detection of Human, Mouse, and Rat JAB1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat JAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6390) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for JAB1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat JAB1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JAB1
JAB1 (Jun activation domain binding protein-1), also called COPS5 or CSN5 (COP9 signalosome-5), is a 334 amino acid (aa), 38 kDa protein that is a subunit of the COP9 signalosome complex. It mediates cytosolic translocation of the cell cycle inhibitory protein, CDKN1B/p27Kip1, for degradation, thus promoting proliferation. It is widely expressed, especially in proliferating cells and up-regulates granulopoiesis when overexpressed. Within the region used as an immunogen, human JAB1 shares 99% aa identity with mouse and rat JAB1. A 379 aa isoform contains 93 alternate aa in place of the N-terminal 48 aa of the 334 aa form.
Long Name
Jun Activation Domain-binding Protein 1
Alternate Names
COPS5, CSN5, MOV-34, SGN5
Gene Symbol
COPS5
UniProt
Additional JAB1 Products
Product Documents for Human/Mouse/Rat JAB1 Antibody
Product Specific Notices for Human/Mouse/Rat JAB1 Antibody
For research use only
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