Human/Mouse/Rat Lyn Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3206
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Lyn
Met1-Ile67
Accession # P07948
Met1-Ile67
Accession # P07948
Specificity
Detects human, mouse, and rat Lyn in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Lyn Antibody
Detection of Human/Mouse/Rat Lyn by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line, PT18 mouse mast/basophil cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Lyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3206) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lyn at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lyn in Human PBMCs.
Lyn was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with monensin using Goat Anti-Human/Mouse/Rat Lyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3206) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human Lyn by Simple WesternTM.
Simple Western lane view shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyn at approximately 58-60 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Lyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3206) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Lyn Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells treated with monensin
Sample: Immersion fixed human peripheral blood mononuclear cells treated with monensin
Simple Western
5 µg/mL
Sample: Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, THP‑1 human acute monocytic leukemia cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Sample: Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, THP‑1 human acute monocytic leukemia cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Western Blot
0.5 µg/mL
Sample: U937 human histiocytic lymphoma cell line, PT18 mouse mast/basophil cell line, and Y3-Ag rat myeloid cell line
Sample: U937 human histiocytic lymphoma cell line, PT18 mouse mast/basophil cell line, and Y3-Ag rat myeloid cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lyn
The nonreceptor tyrosine kinase Lyn (lck/yes-related novel tyrosine kinase) is widely expressed, and the predominant Src family member present in B cells. Activation of Lyn occurs upon its association with cell surface receptors such as BCR and CD40. Analyses from both loss- and gain-of-function mutant mice have revealed that Lyn is an essential regulator of B cell activation, maturation, and tolerance.
Long Name
V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog
Alternate Names
Hck-2, JTK8, v-yes-1
Gene Symbol
LYN
UniProt
Additional Lyn Products
Product Documents for Human/Mouse/Rat Lyn Antibody
Product Specific Notices for Human/Mouse/Rat Lyn Antibody
For research use only
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