Human/Mouse/Rat MEK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2079
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MEK1
Lys2-Val393 (Ser218Asp, Ser222Asp)
Accession # Q02750
Lys2-Val393 (Ser218Asp, Ser222Asp)
Accession # Q02750
Specificity
Detects human, mouse, and rat MEK1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat MEK1 Antibody
Detection of Human/Mouse/Rat MEK1 by Western Blot.
Western blot shows lysates of BT-20 human breast cancer cell line, U937 human histiocytic lymphoma cell line, DU145 human prostate carcinoma cell line, and mouse brain tissue. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse/Rat MEK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MEK1 (5 ng) was included. A specific band for MEK1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat MEK1 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: BT-20 human breast cancer cell line, U937 human histiocytic lymphoma cell line, DU145 human prostate carcinoma cell line, and mouse brain tissue
Sample: BT-20 human breast cancer cell line, U937 human histiocytic lymphoma cell line, DU145 human prostate carcinoma cell line, and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEK1
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
MAP2K1, MKK1
Gene Symbol
MAP2K1
UniProt
Additional MEK1 Products
Product Documents for Human/Mouse/Rat MEK1 Antibody
Product Specific Notices for Human/Mouse/Rat MEK1 Antibody
For research use only
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