Human/Mouse/Rat mGluR5/1a Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS079

R&D Systems, part of Bio-Techne
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PPS079
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all mGluR5/1a Antibodies »

Product Specifications

Immunogen

Peptide from the C-terminus region of rat mGluR5 and mGluR1a

Specificity

Rat ~125 kDa mGluR5 and mGluR1a monomers and ~250 kDa homodimers

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat mGluR5/1a Antibody

Detection of mGluR5/1a antibody by Western Blot

Detection of mGluR5/1a by Western Blot

Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomers and the ~250 kDa dimers of mGluR1a and mGluR5.

Applications for Human/Mouse/Rat mGluR5/1a Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 dilution
Sample: (frozen sections; unpublished observations)

Western Blot

1:1000 dilution
Sample: HEK293 human embryonic kidney cell line expressing mGluR1a and mGluR5
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: mGluR5/1a

Rat metabotropic glutamate receptor 5 (mGluR5b; also mGlu5b) is a 150 kDa, 1183 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. Although both mobilize intracellular Ca++ stores, mGluR1 also induces Ca++ influx via TRP channels. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks cell proliferation. mGluR5 has a large glycosylated extracellular domain (ECD) of 558 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There is one alternate splice form of mGluR5b. It shows a 32 aa deletion between aa 876 - 907 in the cytoplasmic region. The rat mGluR5 C-terminal cytoplasmic region is 90% and 97% aa identical to the equivalent regions in human and mouse mGluR5, respectively.

References

  1. Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
  2. Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
  3. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
  4. Gama, L. et al. (2001) J. Biol. Chem. 276:39053.
  5. Topolnik, L. et al. (2006) J. Physiol. 575:115.

Long Name

Metabotropic Glutamate Receptor 5 and 1

Additional mGluR5/1a Products

Product Documents for Human/Mouse/Rat mGluR5/1a Antibody

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Product Specific Notices for Human/Mouse/Rat mGluR5/1a Antibody

For research use only

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