Human/Mouse/Rat Neurolysin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3814
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Neurolysin
aa 38-704
aa 38-704
Specificity
Detects human, mouse, and rat Neurolysin Neurolysin in Western blots and human Neurolysin in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Meprin alpha, rhMeprin beta subunit, and rhTHOP1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Neurolysin Antibody
Detection of Human, Mouse, and Rat Neurolysin by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Neurolysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3814) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurolysin at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Neurolysin Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Neurolysin (Catalog # 3814-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Neurolysin (Catalog # 3814-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue
Sample: SH‑SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurolysin
Neurolysin, also known as Oligopeptidase M (mitochondrial peptidase), soluble angiotensin II-binding protein and endopeptidase EC 3.4.24.16 (1, 2), is a homologue of Thimet Ologopeptidase (THOP1), a zinc peptidase of the M3 family that also includes mitochondrial intermediate peptidase. Neurolysin expresses in two forms with the difference of an N-terminal transit peptide that targets the localization to mitochondria (1). The shorter form exists in cytosol. The recombinant human Neurolysin is expressed without the transit peptide and is isolated from the cytosol. Like THOP1, Neurolysin is capable of cleaving a number of vasoactive peptides such as bradykinin and neurotensin (3). All known substrates of THOP1 and Neurolysin contain 17 or fewer amino acids.
References
- Serizawa, A. et al. (1995) J. Biol. Chem. 270:2092.
- Barett, A.J. and J.M. Chen (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 356, Elsevier Academic Press, San Diego.
- Norman, M.U. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1978.
Alternate Names
AGTBP, Neurotensin Endopeptidase, NLN, Oligopeptidase M
Gene Symbol
NLN
Additional Neurolysin Products
Product Documents for Human/Mouse/Rat Neurolysin Antibody
Product Specific Notices for Human/Mouse/Rat Neurolysin Antibody
For research use only
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