Human/Mouse/Rat nNOS Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB24162
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 85327
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human nNOS
Ser218-Ser1434
Accession # P29475
Ser218-Ser1434
Accession # P29475
Specificity
Detects human nNos in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat nNOS Antibody
Detection of Mouse and Rat nNOS by Western Blot.
Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat nNOS Monoclonal Antibody (Catalog # MAB24162) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for nNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.nNOS in Human Brain.
nNOS was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human/Mouse/Rat nNOS Monoclonal Antibody (Catalog # MAB24162) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.nNOS in Mouse Spinal Cord.
nNOS was detected in perfusion fixed frozen sections of mouse spinal cord using Mouse Anti-Human/Mouse/Rat nNOS Monoclonal Antibody (Catalog # MAB24162) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat nNOS Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spinal cord and immersion fixed paraffin-embedded human brain (substantia nigra)
Sample: Perfusion fixed frozen sections of mouse spinal cord and immersion fixed paraffin-embedded human brain (substantia nigra)
Western Blot
0.5 µg/mL
Sample: Mouse brain tissue and rat brain tissue
Sample: Mouse brain tissue and rat brain tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: nNOS
Long Name
Neuronal Nitric Oxide Synthase
Alternate Names
NOS1
Gene Symbol
NOS1
UniProt
Additional nNOS Products
Product Documents for Human/Mouse/Rat nNOS Antibody
Product Specific Notices for Human/Mouse/Rat nNOS Antibody
For research use only
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