Human/Mouse/Rat Nucleostemin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1638
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat Nucleostemin
Lys2-Ile538
Accession # Q811S9
Lys2-Ile538
Accession # Q811S9
Specificity
Detects human, mouse, rat Nucleostemin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat Nucleostemin C-terminal peptide (aa 281-538) is observed. Rat Nucleostemin specific IgG was purified by first passing the sera over a rat Nucleostemin aa 2-538 column and then passing the bound fraction over a rat Nucleostemin aa 281-538 column to removed rat Nucleostemin C-terminal specific IgG.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Nucleostemin Antibody
Detection of Human Nucleostemin by Western Blot.
Western blot shows lysates of PC-3 human prostate cancer cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nucleostemin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Nucleostemin in U2OS Human Cell Line.
Nucleostemin was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Nucleostemin by Simple WesternTM.
Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Nucleostemin at approximately 83 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Nucleostemin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line
Sample: Immersion fixed U2OS human osteosarcoma cell line
Simple Western
5 µg/mL
Sample: PC‑3 human prostate cancer cell line
Sample: PC‑3 human prostate cancer cell line
Western Blot
0.25 µg/mL
Sample: PC3 human prostate cancer cell line and SW480 human colon adenocarcinoma cell line
Sample: PC3 human prostate cancer cell line and SW480 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nucleostemin
References
- Tsai, R.Y. and R.D. McKay (2002) Genes Dev. 16:2991.
- Baddoo, M. et al. (2003) J. Cell Biochem. 89:1235.
- Normile, D. (2002) Science 298:1869.
Alternate Names
GNL3
Gene Symbol
GNL3
UniProt
Additional Nucleostemin Products
Product Documents for Human/Mouse/Rat Nucleostemin Antibody
Product Specific Notices for Human/Mouse/Rat Nucleostemin Antibody
For research use only
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