Human/Mouse/Rat Park7/DJ-1 Antibody

Detection of Human, Mouse, and Rat Park7/DJ‑1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MEF mouse embryonic feeder cells, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Park7/DJ-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Park7/DJ‑1 in HeLa Human Cell Line.

Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Shows Human Park7/DJ‑1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.