Human/Mouse/Rat Parvalbumin alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5058
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Parvalbumin alpha
Ser2-Ser110
Accession # P20472
Ser2-Ser110
Accession # P20472
Specificity
Detects human, mouse, and rat Parvalbumin alpha in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Parvalbumin alpha Antibody
Detection of Human/Mouse/Rat Parvalbumin alpha by Western Blot.
Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Parvalbumin a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Parvalbumin alpha in Human Brain.
Parvalbumin a was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 5 µg/mL Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Rat Parvalbumin alpha by Simple WesternTM.
Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Parvalbumin a at approximately 15 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Parvalbumin alpha Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Simple Western
50 µg/mL
Sample: Rat brain tissue, mouse brain (cortex), and human brain (cerebellum)
Sample: Rat brain tissue, mouse brain (cortex), and human brain (cerebellum)
Western Blot
1 µg/mL
Sample: Human, mouse and rat brain tissue
Sample: Human, mouse and rat brain tissue
Reviewed Applications
Read 1 review rated 5 using AF5058 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Parvalbumin alpha
Parvalbumin (Parvalbumin alpha) is a 12 kDa member of the parvalbumin family of Ca++-binding proteins. In human, it is expressed in intrafusal muscle fibers, plus GABAergic interneurons and cerebellar Purkinje and basket cells. It presumably acts as a Ca++ buffer that shortens the duration of fiber contraction. Human Parvalbumin is 110 amino acids (aa) in length. It contains two EF-hand domains (aa 39-74 and 78-110) that bind calcium. There are three potential isoform variants. One shows an alternate start site at Met33, a second shows a six aa substitution for the C-terminal nine amino acids and a third shows a deletion of Gly99-Val100. Human Parvalbumin alpha is 51% aa identical to human Parvalbumin beta and is 87% plus 92% aa identical to mouse and rat Parvalbumin, respectively.
Alternate Names
PVALB
Gene Symbol
PVALB
UniProt
Additional Parvalbumin alpha Products
Product Documents for Human/Mouse/Rat Parvalbumin alpha Antibody
Product Specific Notices for Human/Mouse/Rat Parvalbumin alpha Antibody
For research use only
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