Human/Mouse/Rat PBEF/Visfatin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB40441

R&D Systems, part of Bio-Techne
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MAB40441
MAB40441-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 882104

View all PBEF/Visfatin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PBEF/Visfatin
Pro27-His491
Accession # P43790

Specificity

Detects human PBEF/Visfatin in direct ELISAs and Western blots. 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat PBEF/Visfatin Antibody

Detection of Human, Mouse, and Rat PBEF/Visfatin antibody by Western Blot.

Detection of Human, Mouse, and Rat PBEF/Visfatin by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/ macrophage cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PBEF/Visfatin antibody by Western Blot.

Detection of Human PBEF/Visfatin by Western Blot.

Western blot shows lysates of human heart tissue, 293T human embryonic kidney cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/ Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PBEF/Visfatin antibody by Western Blot.

Detection of Human PBEF/Visfatin by Western Blot.

Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/ Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Applications for Human/Mouse/Rat PBEF/Visfatin Antibody

Application
Recommended Usage

Simple Western

5-50 µg/mL
Sample: KG‑1 human acute myelogenous leukemia cell line, HepG2 human hepatocellular carcinoma cell line, HEK293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and Neuro‑2A mouse neuroblastoma cell line

Western Blot

0.5-1 µg/mL
Sample: 293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, Neuro‑2A mouse neuroblastoma cell line, Rat‑2 rat embryonic fibroblast cell line human heart tissue, A431 human epithelial carcinoma cell line, KG‑1 human acute myelogenous leukemia cell line, and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Gene Symbol

NAMPT

UniProt

P43790

Additional PBEF/Visfatin Products

Product Documents for Human/Mouse/Rat PBEF/Visfatin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PBEF/Visfatin Antibody

For research use only

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