Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

Bio-Techne includes R&D Systems | Catalog # AF869

Discontinued Product
AF869 has been discontinued. View all p38 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Simple Western, Western Blot

Cited:

Blocking, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all p38 Antibodies »

Product Summary for Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

Immunogen

Phosphopeptide containing human, rat and mouse p38 alpha T180/Y182 site

Specificity

Detects human, mouse and rat Phospho-p38 alpha MAP Kinase when dually phosphorylated at T180/Y182. May also recognize the related phosphorylated isoforms p38 beta, p38 gamma and p38 delta.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

Detection of Human Phospho-p38 MAP Kinase (T180/Y182) antibody by Western Blot.

Detection of Human Phospho-p38 MAP Kinase (T180/Y182) by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IL-1 beta (Catalog # 201-LB) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/ Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-p38 (T180/Y182) antibody in Mouse Embryo (13 d by Immunohistochemistry (IHC-Fr).p by Immunohistochemistry (IHC-Fr).c by Immunohistochemistry (IHC-Fr).) by Immunohistochemistry (IHC-Fr).

Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.).

p38 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human Phospho-p38 MAP Kinase (T180/Y182) antibody by Simple WesternTM.

Detection of Human Phospho-p38 MAP Kinase (T180/Y182) by Simple WesternTM.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with ultraviolet light (UV), loaded at 0.5 mg/mL. A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.) 

Simple Western

5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with ultraviolet light (UV)

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human IL-1 beta (Catalog # 201-LB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF869 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Product Documents for Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

For research use only

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