Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5734

R&D Systems, part of Bio-Techne
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AF5734
AF5734-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all PI 3-Kinase p55 gamma Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3-Kinase p55 gamma
Lys251-Gly378
Accession # Q92569

Specificity

Detects endogenous human, mouse and rat PI 3-Kinase p55 gamma in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Detection of Human/Mouse/Rat PI 3-Kinase p55? antibody by Western Blot.

Detection of Human/Mouse/Rat PI 3-Kinase p55 gamma by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3-Kinase p55 gamma at approximately 55 to 57 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p55 gamma

p55 gamma (Protein of 55 kDa, gamma subunit; also PI 3-Kinase regulatory subunit gamma and p55PIK) is a 55-57 kDa member of the PI 3K p85 family of regulatory subunits. It is widely expressed and serves as a recruitment subunit for the p110 catalytic subunit of PI 3-Kinase. p55 gamma/p55PIK should not be confused with the 55 kDa alpha splice variant of the p85 alpha gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length and contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452).

Long Name

PI 3-Kinase p55 gamma

Alternate Names

p55PIK, PI 3Kinase p55 gamma, PIK3R3

Entrez Gene IDs

8503 (Human); 18710 (Mouse); 60664 (Rat)

Gene Symbol

PIK3R3

UniProt

Q92569 (Human)

Additional PI 3-Kinase p55 gamma Products

Product Documents for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

For research use only

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