Human/Mouse/Rat PKA C Pan Specific Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4175
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKA C gamma
Met1-Phe351
Accession # P22612
Met1-Phe351
Accession # P22612
Specificity
Detects recombinant human PKA C alpha, C beta, and C gamma, and endogenous human PKA C isoforms in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PKA C Pan Specific Antibody
Detection of Human PKA C by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell lines . PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PKA C Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4175) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKA C Pan Specific at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PKA C Pan Specific Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell lines
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell lines
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF4175 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA C (pan)
The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit
Alternate Names
CAFD1, PKACA, PPNAD4
UniProt
Additional PKA C (pan) Products
Product Documents for Human/Mouse/Rat PKA C Pan Specific Antibody
Product Specific Notices for Human/Mouse/Rat PKA C Pan Specific Antibody
For research use only
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