Human/Mouse/Rat PKC iota/ lambda/ zeta Antibody

Detection of Human, Mouse, and Rat PKC iota/ lambda/ zeta by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, DU145 human prostate carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKC iota/lambda/zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human PKC iota, PKC zeta, and PKC alpha (2 ng/lane) were included. Specific bands were detected at approximately 22 kDa for recombinant PKC iota/zeta and ~80 kDa for natural PKC iota/zeta (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PKC iota/ lambda/ zeta in Human Pancreas.

PKC iota/lambda/zeta was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human/Mouse/Rat PKC iota/lambda/zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.

Detection of Human PKC iota/ lambda/ zeta by Simple Western^TM.

Simple Western shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for PKC iota/ lambda/ zeta at approximately 80 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKC iota/ lambda/ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.