Human/Mouse/Rat PKM1/2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7244
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKM1/2
Ser434-Pro531
Accession # P14618
Ser434-Pro531
Accession # P14618
Specificity
Detects human, mouse, and rat PKM2 in Western blots and detects recombinant human PKM1/2 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PKM1/2 Antibody
Detection of Human, Mouse, and Rat PKM2 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.PKM2 in Wi-38 Human Cell Line.
PKM2 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.PKM2 in RAW 264.7 Mouse Cell Line.
PKM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat PKM1/2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed Wi-38 human lung fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line
Sample: Immersion fixed Wi-38 human lung fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse and rat testis
Sample: Perfusion fixed frozen sections of mouse and rat testis
Knockout Validated
PKM2 is specifically detected in HeLa human
cervical epithelial carcinoma parental cell line but is not detectable in
PKM2 knockout HeLa cell line.
Simple Western
2 µg/mL
Sample: A549 human lung carcinoma cell line
Sample: A549 human lung carcinoma cell line
Western Blot
0.2 µg/mL
Sample: A549 human lung carcinoma cell line, KG‑1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, NRK rat normal kidney cell line, U‑87 MG human glioblastoma/astrocytoma cell line, HeLa human cervical epithelial carcinoma cell line, human skeletal muscle
Sample: A549 human lung carcinoma cell line, KG‑1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, NRK rat normal kidney cell line, U‑87 MG human glioblastoma/astrocytoma cell line, HeLa human cervical epithelial carcinoma cell line, human skeletal muscle
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKM2
aa 389‑433 of PKM2. This variant shows limited expression (striated muscle) and hyperbolic Michaelis-Menten kinetics. There are additional isoform variants of PKM2 that show either a deletion of aa 59‑132, or a 67 aa substitution for aa 1‑82. Over aa 434‑531, human PKM2 shares 95% aa sequence identity with mouse PKM2.
Long Name
Pyruvate Kinase, Muscle
Alternate Names
CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK
Gene Symbol
PKM
UniProt
Additional PKM2 Products
Product Documents for Human/Mouse/Rat PKM1/2 Antibody
Product Specific Notices for Human/Mouse/Rat PKM1/2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...