Human/Mouse/Rat PLC-beta 4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5128
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PLC-beta 4
Lys75-Gln250
Accession # Q15147
Lys75-Gln250
Accession # Q15147
Specificity
Detects endogenous human, mouse, and, rat PLC-beta 4 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PLC-beta 4 Antibody
Detection of Human/Mouse/Rat PLC-beta 4 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5128) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PLC-beta 4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PLC-beta 4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: DU145 human prostate carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and Neuro-2A mouse neuroblastoma cell line
Sample: DU145 human prostate carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and Neuro-2A mouse neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-beta 4
PLC-beta 4 (phospholipase C-beta 4; also PLCretA) is a 130 kDa member of the beta-type PLC isozyme family. It is notably expressed in cerebellum and retina. PLC-beta 4 is associated with rhodopsin family GPCRs, where activation of Gq alpha subunits initiates PLC-beta 4 hydrolysis of PIP2. Human PLC-beta 4 is 1175 amino acids (aa) in length. It contains a catalytic X-box (aa 313‑463) and Y-box (aa 565‑681), followed by a calcium-binding C2 domain (aa 688‑786). There are multiple isoforms. One shows an alternate start site at Met114, a second contains a 40 aa substitution for the C-terminal 22 aa, a third shows a 14 aa substitution for the N-terminal 167 aa, and other forms demonstrate combinations of these alternative splice forms. Over aa 75‑250, human PLC-beta 4 is 97% aa identical to mouse PLC-beta 4.
Long Name
Phospholipase C beta 4
Alternate Names
PLCB4, PLCbeta 4
Gene Symbol
PLCB4
UniProt
Additional PLC-beta 4 Products
Product Documents for Human/Mouse/Rat PLC-beta 4 Antibody
Product Specific Notices for Human/Mouse/Rat PLC-beta 4 Antibody
For research use only
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