Human/Mouse/Rat PLC-gamma 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3288
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PLC-gamma 1
Ser1091-Leu1290
Accession # P19174
Ser1091-Leu1290
Accession # P19174
Specificity
Detects human, mouse, and rat PLC-gamma 1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PLC-gamma 1 Antibody
Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat PLC- gamma1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PLC-gamma 1 by Simple Western
TIM-3 Association with Intracellular Kinases in CD8+/MART-1+ T cells.TIM-3 co-immunoprecipitation analysis of unactivated and 15 min. stimulation with anti-CD3/CD28 beads (activated). Equivalent amounts of protein (~2mg) were co-immunoprecipitated with pAb anti-TIM-3 antibody and western blot was performed using capillary electrophoresis. Cleared lysate served as a loading control for individual antibody reactivity. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140694), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse/Rat PLC-gamma 1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line
Sample: HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 1
Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.
Long Name
Phospholipase C gamma 1
Alternate Names
PLCG1, PLCgamma 1, PPLCA
Gene Symbol
PLCG1
UniProt
Additional PLC-gamma 1 Products
Product Documents for Human/Mouse/Rat PLC-gamma 1 Antibody
Product Specific Notices for Human/Mouse/Rat PLC-gamma 1 Antibody
For research use only
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