Human/Mouse/Rat Plexin A2 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5486A

R&D Systems, part of Bio-Techne
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FAB5486A
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AF5486
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

View all Plexin A2 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2
Met35-Pro1237
Accession # P70207

Specificity

Detects mouse and rat Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A3, and recombinant human Plexin B1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Plexin A2 APC-conjugated Antibody

Detection of Plexin A2 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Plexin A2 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Plexin A2 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry.

Detection of Plexin A2 in PC‑12 Rat Cell Line by Flow Cytometry.

PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Plexin A2 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of Plexin A2 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse/Rat Plexin A2 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line, PC‑12 rat adrenal pheochromocytoma cell line, and HUVEC human umbilical vein endothelial cells


Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Plexin A2

Plexin A2 (also OCT) is a 230-250 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, CD31+ LYVE+ lymphoid endothelial cells, osteoblasts, and cardiac neural crest cells. It blocks neurite outgrowth and lymphatic capillary formation, but promotes cell migration and osteogenesis, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilins for Sema3A and C. Notably, it would appear to contribute to the receptor complex for BMPs by interacting with both BMPR1A/ALK3 and BMPR1B/ALK6 in conjunction with BMPR2. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane homodimeric glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.

Alternate Names

OCT, PLXN2, PLXNA2

Entrez Gene IDs

5362 (Human); 18845 (Mouse); 289392 (Rat)

Gene Symbol

PLXNA2

UniProt

P70207

Additional Plexin A2 Products

Product Documents for Human/Mouse/Rat Plexin A2 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Plexin A2 APC-conjugated Antibody

For research use only

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