Human/Mouse/Rat PP2C beta/PPM1B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4396
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PP2C beta
Met1-Gly378
Accession # O75688
Met1-Gly378
Accession # O75688
Specificity
Detects endogenous human, mouse and rat PP2C beta in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PP2C alpha or PP2C delta.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PP2C beta/PPM1B Antibody
Detection of Human/Mouse/Rat PP2C beta/PPM1B by Western Blot.
Western blot shows lysates of CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP2C beta/PPM1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PP2C beta/PPM1B at approximately 53 and 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PP2C beta/PPM1B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line
Sample: CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C beta/PPM1B
Protein phosphatase 2C beta (PP2C beta), also called protein phosphatase, magnesium-dependent type 1B (PPM1B), preferentially dephosphorylates serine and threonine residues on proteins. There are five splice variants with sizes of 53, 43, 42, 36, and 21 kDa. This enzyme is resistant to inhibitors of other protein phosphatases such as okadaic acid and calyculin. Association of PP2C beta with I kappaB kinase inhibits its phosphorylating activity and is regulated by TNFalpha stimulation. PP2C beta is found in many cell types and tissues, especially muscle. The mRNA for PP2C beta accumulates in mature spermatocytes and metaphase-arrested, unfertilized oocytes. Knockout of PP2C beta in mice is lethal prior to embryo implantation.
Long Name
Protein Phosphatase 2C beta
Alternate Names
Pp2c2, PP2CB, PPM1B
Gene Symbol
PPM1B
UniProt
Additional PP2C beta/PPM1B Products
Product Documents for Human/Mouse/Rat PP2C beta/PPM1B Antibody
Product Specific Notices for Human/Mouse/Rat PP2C beta/PPM1B Antibody
For research use only
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