Human/Mouse/Rat Prohibitin 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4794
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Prohibitin 2
Lys89-Lys299
Accession # Q99623
Lys89-Lys299
Accession # Q99623
Specificity
Detects human, mouse and rat Prohibitin 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Prohibitin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Prohibitin 2 Antibody
Detection of Human Prohibitin 2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Prohibitin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4794) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prohibitin 2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Mouse/Rat Prohibitin 2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line
Sample: HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prohibitin 2
Prohibitin 2 (PHB2; also B-cell receptor-associated protein BAP37 and REA) is a 37 kDa, ubiquitous member of the prohibitin family of molecules. PHB2 is found in multiple intracellular compartments. It is suggested to be a component of an inner mitochondrial membrane complex and a nuclear repressor of the estrogen receptor (ER). Human PHB2 is 299 amino acids (aa) in length. It contains a mitochondrial localization signal (aa 1‑50), two ER repressor domains (aa 19‑49 and aa 150‑174), and a central coiled-coil domain (aa 190‑238). There may be one splice variant that shows a premature truncation after aa 289. Over aa 89‑299, mouse and rat PHB2 are 100% identical to human PHB2.
Alternate Names
BAP, Bap37, BCAP37, D-Prohibitin, p22, PHB2, PNAS-141, REA
Gene Symbol
PHB2
UniProt
Additional Prohibitin 2 Products
Product Documents for Human/Mouse/Rat Prohibitin 2 Antibody
Product Specific Notices for Human/Mouse/Rat Prohibitin 2 Antibody
For research use only
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