Human/Mouse/Rat Protocadherin-17 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7669
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human Protocadherin-17
Leu18-Ser705
Accession # O14917
Leu18-Ser705
Accession # O14917
Specificity
Detects human, mouse and rat Protocadherin-17 in Western blots. In direct ELISAs, less than 5% cross-reactivity with Protocadherin-10 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Protocadherin-17 Antibody
Detection of Mouse and Rat Protocadherin-17 by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7669) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Protocadherin-17 at approximately 120-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Protocadherin-17 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse brain (cortex) tissue and rat brain (cerebellum) tissue
Sample: Mouse brain (cortex) tissue and rat brain (cerebellum) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protocadherin-17
Protocadherin 17 (PCDH-17) is a 120-150 kDa glycoprotein, delta2-group member of the of the protocadherin subfamily, cadherin family of molecules. It is expressed on endothelium and epithelium such as stratified squamous epithelium and (likely) gastric columnar epithelium. PCDH17 is posited to act as an adhesion molecule, and appears to play a role in promoting cell cycle arrest and inhibiting migration. Mature human PCDH-17 is a type I transmembrane protein that is 1142 amino acids (aa) in length. It contains a 690 aa extracellular domain (ECD) (aa 18-707) plus a 431 aa cytoplasmic region. There are 6 cadherin domains in the ECD (aa 18-695). Two splice forms are reported that involve substitutions in the cytoplasmic region. The first possesses a 14 aa substitution, while the second shows a 25 aa substitution, both for aa 876-1159. Over aa 18-705, human PCDH-17 shares 98% aa identity with mouse PCDH-17.
Alternate Names
PCDH17, PCDH68, PCH68, Protocadherin17
Gene Symbol
PCDH17
UniProt
Additional Protocadherin-17 Products
Product Documents for Human/Mouse/Rat Protocadherin-17 Antibody
Product Specific Notices for Human/Mouse/Rat Protocadherin-17 Antibody
For research use only
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