Human/Mouse/Rat RACK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3434
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RACK1
Met1-Arg317
Accession # P63244
Met1-Arg317
Accession # P63244
Specificity
Detects human, mouse, and rat RACK1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat RACK1 Antibody
Detection of Human/Mouse/Rat RACK1 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A20 mouse B cell lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RACK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3434) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RACK1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RACK1 in HeLa Human Cell Line.
RACK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat RACK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3434) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat RACK1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, A20 mouse B cell lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: HT-29 human colon adenocarcinoma cell line, A20 mouse B cell lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RACK1
Long Name
Receptor for Activated Protein Kinase C 1
Alternate Names
GNB2L1
Gene Symbol
RACK1
UniProt
Additional RACK1 Products
Product Documents for Human/Mouse/Rat RACK1 Antibody
Product Specific Notices for Human/Mouse/Rat RACK1 Antibody
For research use only
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