Human/Mouse/Rat Slit3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3629

R&D Systems, part of Bio-Techne
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AF3629
AF3629-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Neutralization

Cited:

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Slit3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Slit3
Ser27-His901
Accession # Q9WVB4

Specificity

Detects human, mouse and rat Slit3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Mouse/Rat Slit3 Antibody

Slit3 in 13 D.P.C. Mouse Embryo.

Slit3 was detected in immersion fixed paraffin-embedded sections of 13 d.p.c. mouse embryo using Goat Anti-Human/Mouse/Rat Slit3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3629) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing braiin and cartillage.

Applications for Human/Mouse/Rat Slit3 Antibody

Application
Recommended Usage

Immunohistochemistry

5 µg/mL
Sample: Immersion-fixed paraffin-embedded 13 d.p.c. mouse embryo

Neutralization

In a functional ELISA, 3-18 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB) to immobilized Recombinant Mouse Slit3 (Catalog # 9296-SL) coated at 500 ng/mL (100 µL/well). At 25 µg/mL, this antibody will block >80% of the binding.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Slit3

Slit3 is a member of the slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1). Like other mammalian family members, the 1523 amino acid (aa) Slit3 contains a signal sequence followed by 23 leucine-rich repeats (LRR) and nine EGF-like sequences (1). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7, and a C-terminal cysteine-rich domain (1). In Drosophila Slit, specific LRR are sites of ROBO interaction and homodimerization (2). Drosophila Slit shows equal similarities with all three mouse slit proteins, which are 59‑66% identical with each other (1). During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in function (3). Slit3 is also expressed in the lung, kidney, skeletal muscle and heart, both during development and postnatally (1, 4‑6). ROBO2 is often found in complementary areas (4). Mice with genetically disrupted Slit3 have thin diaphragms with disorganized collagen fibrils and frequently develop diaphragmatic hernias (5, 6). Abnormalities in kidney development may also occur (5). Although Slit proteins are generally considered to be secreted, significant amounts of Slit3 may be retained in the mitochondria because of features in the signal sequence that indicate a high probability of mitochondrial targeting (7). Secreted Slit is often membrane-associated (7). Mouse Slit3 shows 98% and 94% amino acid identity with rat and human Slit3, respectively.

References

  1. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  2. Howitt, J. A. et al. (2004) EMBO J. 23:4406.
  3. Long, H. et al. (2004) Neuron 42:213.
  4. Greenberg, J. M. et al. (2004) Dev. Dyn. 230:350.
  5. Liu, J. et al. (2003) Mech. Dev. 120:1059.
  6. Yuan, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5217.
  7. Little, M. H. et al. (2001) Am. J. Physiol. Cell Physiol. 281:C486.

Long Name

SLIT Homolog 3

Alternate Names

MEGF5, SLIL2

Entrez Gene IDs

6586 (Human); 20564 (Mouse); 83467 (Rat)

Gene Symbol

SLIT3

UniProt

Q9WVB4

Additional Slit3 Products

Product Documents for Human/Mouse/Rat Slit3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Slit3 Antibody

For research use only

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