Human/Mouse/Rat SMC1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3294
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SMC1
Met837-Gln1233
Accession # Q14683.2
Met837-Gln1233
Accession # Q14683.2
Specificity
Detects human, mouse, and rat SMC1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat SMC1 Antibody
Detection of Human SMC1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human SMC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3294) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SMC1 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat SMC1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMC1
Structural Maintenance of Chromosome 1 (SMC1) is an ATPase which, with SMC3, forms a heterodimeric complex. These dimers form a V-shaped molecule with two long coiled-coil arms, each having an ATP-binding domain at its distal end. The SMC1/SMC3 heterodimer plays an important role in sister chromatid cohesion during metaphase, associates with the nuclear matrix and centrosome during interphase and localizes to kinetochores, spindles and spindle poles during mitosis. Smc1 is also a component of the DNA damage response and participates in the cellular response to DNA damage through its phosphorylation on Ser 957 and 966.
Long Name
Structural Maintenance of Chromosomes 1
Alternate Names
SB1.8, SMC1A, SMC1L1
Gene Symbol
SMC1A
UniProt
Additional SMC1 Products
Product Documents for Human/Mouse/Rat SMC1 Antibody
Product Specific Notices for Human/Mouse/Rat SMC1 Antibody
For research use only
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