Human/Mouse/Rat SOD2/Mn-SOD Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3419
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SOD2
Lys25-Lys222
Accession # P04179
Lys25-Lys222
Accession # P04179
Specificity
Detects human, mouse and rat SOD2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat SOD2/Mn-SOD Antibody
Detection of Human/Mouse/Rat SOD2/Mn-SOD by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOD2/Mn-SOD at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Human and Mouse SOD2/Mn‑SOD by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD2/Mn-SOD at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat SOD2/Mn-SOD Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOD2/Mn-SOD
Long Name
Superoxide Dismutase-2
Alternate Names
IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial
Gene Symbol
SOD2
UniProt
Additional SOD2/Mn-SOD Products
Product Documents for Human/Mouse/Rat SOD2/Mn-SOD Antibody
Product Specific Notices for Human/Mouse/Rat SOD2/Mn-SOD Antibody
For research use only
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