Human/Mouse/Rat Src Antibody

Src in Human Liver Cancer Tissue.

Src was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Src in Human Liver.

Src was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Src by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Src at approximately 62 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Src by Western Blot

Lasp1 affects tissue architecture in the synovium of hTNFtg mice.a H&E stainings (scale bar: 50 µm) of paraffin sections from hind paws from 12 weeks old wt, Lasp1−/−, hTNFtg and hTNFtg/Lasp1−/− female mice (n = 3 in each group). Evaluation of synovial cell density was performed by counting cell numbers per high power field (HPF, 10.000 µm2) (n = 10, 3 HPF/animal). Data presented as mean ± SEM, *P < 0.05, **P < 0.001, ***P < 0.0001 (two-tailed Mann–Whitney U test). b Immunofluorescence staining for PDPN, FAP alpha, THY1 expression and the nuclei with DAPI in white in 12 weeks old joints (representative images of n = 5 per group, scale bar: 10 µm). c Immunoblotting and quantification for phospho-specific Src kinase (pSrc418) and total Src kinase expression as well as for phospho-specific AKT kinase (pAKT) and total AKT in FLS from hTNFtg and hTNFtg/Lasp1−/− mice stimulated with recombinant murine PDGF for the indicated time periods (n = 3 each group). Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34131132), licensed under a CC-BY license. Not internally tested by R&D Systems.