Human/Mouse/Rat TDP-43/TARDBP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB77781

R&D Systems, part of Bio-Techne
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MAB77781-100
MAB77781-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 982022

View all TDP-43/TARDBP Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human TDP-43/TARDBP
Met1-Thr103
Accession # Q13148

Specificity

Detects human TDP-43/TARDBP in direct ELISAs. Detects human, mouse, and rat TDP-43/TARDBP in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat TDP-43/TARDBP Antibody

Detection of Human, Mouse, and Rat TDP-43/TARDBP antibody by Western Blot.

Detection of Human, Mouse, and Rat TDP-43/TARDBP by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB77781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TDP-43/TARDBP at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TDP-43/TARDBP antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

TDP-43/TARDBP in Mouse Splenocytes.

TDP-43/TARDBP was detected in immersion fixed mouse splenocytes using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB77781) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
TDP-43/TARDBP antibody in Human Brain by Immunohistochemistry (IHC-P).

TDP-43/TARDBP in Human Brain.

TDP-43/TARDBP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB77781) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat TDP-43/TARDBP Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line

Reviewed Applications

Read 1 review rated 5 using MAB77781 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDP-43/TARDBP

TDP-43 (TAR DNA binding protein of 43 kDa; gene name TARDBP) is a 414 amino acid (aa) nuclear RNA/DNA binding phosphoprotein that regulates transcription and splicing. TDP-43 is aberrantly expressed in most forms of amylotrophic lateral sclerosis and frontotemporal lobar degeneration. In these cases it is absent from the nucleus and accumulates, along with a truncated 20-28 kDa form, in ubiquitin-containing inclusion bodies. TDP-43 can also influence CFTR exon 9 skipping in cystic fibrosis. Within aa 1-103, human and mouse TDP-43 share 100% aa sequence identity, and 99% aa identity with rat TDP-43.

Long Name

TAR DNA-binding Protein 43

Alternate Names

ALS10, TARDBP, TDP43

Entrez Gene IDs

23435 (Human)

Gene Symbol

TARDBP

UniProt

Q13148

Additional TDP-43/TARDBP Products

Product Documents for Human/Mouse/Rat TDP-43/TARDBP Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat TDP-43/TARDBP Antibody

For research use only

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