Human/Mouse/Rat Thymosin  beta10 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6429

R&D Systems, part of Bio-Techne
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AF6429
AF6429-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Thymosin beta 10 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Thymosin beta10
Ala2-Ser44
Accession # P63313

Specificity

Detects human Thymosin beta10 in direct ELISAs. Detects human, mouse, and rat Thymosin beta10 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Thymosin beta4 and rhThymosin beta16 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Thymosin  beta10 Antibody

Detection of Human, Mouse, and Rat Thymosin beta10 antibody by Western Blot.

Detection of Human, Mouse, and Rat Thymosin beta10 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, NTera-2 human testicular embryonic carcinoma cell line, C6 rat glioma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Thymosin beta10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6429) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta10 at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Thymosin  beta10 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, NTera‑2 human testicular embryonic carcinoma cell line, C6 rat glioma cell line, and Balb/3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thymosin beta 10

Thymosin beta 10 (T beta10) is a 6.5 kDa member of the beta-thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 5.0-7.0. Multiple cell types produce T beta10, either constitutively, or after stimulation. They include monocytes/macrophages, endothelial cells, thyroid epithelium, spermatids, fetal cerebellar Purkinje cells plus renal tubule epithelium, and multiple tumor types. T beta10 is principally an intracellular molecule. It forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration.  It is also known to be noncanonically secreted, acting on endothelium and blocking angiogenesis. Mature human T beta10 is 43 aa in length (aa 2-44) (SwissProt #:P63313). It contains an actin-binding site (aa 18-23), an N-terminal acetylated alanine, four acetylated lysines (#4; 15; 26; 39), and one phosphorylation site at Thr23. Mature T beta10 is identical to mouse T beta10 in aa sequence, and it shares 74% aa sequence identity with its human family member T beta4.

Alternate Names

MIG12, PTMB10, TB10, THYB10, TMSB10

Entrez Gene IDs

9168 (Human); 19240 (Mouse)

Gene Symbol

TMSB10

UniProt

P63313 (Human)

Additional Thymosin beta 10 Products

Product Documents for Human/Mouse/Rat Thymosin  beta10 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Thymosin  beta10 Antibody

For research use only

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