Human/Mouse/Rat UCH-L3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6008
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human UCH‑L3
Glu2-Ala230
Accession # P15374
Glu2-Ala230
Accession # P15374
Specificity
Detects human, mouse, and rat UCH‑L3 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat UCH-L3 Antibody
Detection of Human, Mouse, and Rat UCH‑L3 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat UCH-L3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UCH-L3 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2X.Applications for Human/Mouse/Rat UCH-L3 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Sample: HEK293 human embryonic kidney cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UCH-L3
UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH‑L3 cleaves both monomeric ubiquitin (Ub) from Ub‑protein conjugates, and a GGLRQ peptide from the C‑terminus of the Ub‑like protein NEDD‑8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179‑230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH‑L3.
Long Name
Ubiquitin C-terminal Hydrolase L3
Alternate Names
Ubiquitin Thioesterase L3, UCHL3
Gene Symbol
UCHL3
UniProt
Additional UCH-L3 Products
Product Documents for Human/Mouse/Rat UCH-L3 Antibody
Product Specific Notices for Human/Mouse/Rat UCH-L3 Antibody
For research use only
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