Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4815
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Vang-like Protein 2/VANGL2
Gln241-Val521
Accession # Q9ULK5
Gln241-Val521
Accession # Q9ULK5
Specificity
Detects human, mouse, and rat Vang-like Protein 2/VANGL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VANGL1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody
Detection of Human/Mouse/Rat Vang-like Protein 2/ VANGL2 by Western Blot.
Western blot shows lysates of mouse kidney, human and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vang-like Protein 2/VANGL2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Vang-like Protein 2/VANGL2 in Mouse Embryo.
Vang-like Protein 2/VANGL2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15.5)
Sample: Immersion fixed frozen sections of mouse embryo (E15.5)
Western Blot
1 µg/mL
Sample: Mouse kidney, human and rat brain tissue
Sample: Mouse kidney, human and rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vang-like Protein 2/VANGL2
VANGL2 (van Gogh-like protein 2; also Strabismus and Ltap) is a 65 kDa member of the Vang family of proteins. It is found in the membranes of embryonic neurons and epithelium where it associates with DVL1-3 and various frizzleds. VANGL2 mutations are associated with neural tube defects. Human VANGL2 is a 521 amino acid (aa) 4-transmembrane protein that contains an N-terminal (aa 1‑108) and C-terminal (aa 239‑521) cytoplasmic domain. There is a Ser-rich region in the N-terminus (aa 5‑20) and a PDZ-binding motif over aa 506‑521. Over aa 241‑521, human, mouse and rat show total identity in amino acid sequence.
Alternate Names
LPP1, LTAP, STB1, STBM, Strabismus, Vang like Protein 2, VANGL2
Gene Symbol
VANGL2
UniProt
Additional Vang-like Protein 2/VANGL2 Products
Product Documents for Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody
Product Specific Notices for Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody
For research use only
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