Human/Mouse/Rat XPD Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3296
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human XPD
Met1-Gly360
Accession # P18074
Met1-Gly360
Accession # P18074
Specificity
Detects human, mouse, and rat XPD in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat XPD Antibody
Detection of Human/Mouse/Rat XPD by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat XPD Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XPD
The Xeroderma Pigmentosum D (XPD) protein is a DNA helicase that acts as a subunit of the RNA polymerase II complex during nucleotide excision repair (NER) and transcription-coupled repair (TCR). XPD is also involved in p53-mediated apoptosis. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.
Long Name
Xeroderma Pigmentosum D
Alternate Names
EM9, ERCC2
Gene Symbol
ERCC2
UniProt
Additional XPD Products
Product Documents for Human/Mouse/Rat XPD Antibody
Product Specific Notices for Human/Mouse/Rat XPD Antibody
For research use only
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