Human/Mouse/Rat YAP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8094
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
CyTOF-reported, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 867711
Product Specifications
Immunogen
E. coli-derived recombinant human YAP1
Ala347-Leu504
Accession # P46937
Ala347-Leu504
Accession # P46937
Specificity
Detects human YAP1 in ELISA. Detects human, mouse and rat YAP1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat YAP1 Antibody
Detection of Human, Mouse, and Rat YAP1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # MAB8094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YAP1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
YAP1 in Human Prostate.
YAP1 was detected in formalin fixed paraffin-embedded sections of human prostate using Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # MAB8094) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human YAP1 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for YAP1 at approximately 79 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat YAP1 Monoclonal Antibody (Catalog # MAB8094) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat YAP1 Antibody
Application
Recommended Usage
CyTOF-reported
Brodie, T.M. et al. (2018) Cytometry Part
A. 93: 406. Ready to be labeled using established
conjugation methods. No BSA or other carrier proteins that could interfere with
conjugation.
Immunohistochemistry
8-25 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human prostate
Sample: Formalin fixed paraffin-embedded sections of human prostate
Knockout Validated
YAP1
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
YAP1 knockout HeLa cell line.
Simple Western
20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line
Reviewed Applications
Read 1 review rated 5 using MAB8094 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: YAP1
Long Name
Yes-associated Protein 1
Alternate Names
YAP2, YAP65, YKI, Yorkie Homolog
Gene Symbol
YAP1
UniProt
Additional YAP1 Products
Product Documents for Human/Mouse/Rat YAP1 Antibody
Product Specific Notices for Human/Mouse/Rat YAP1 Antibody
For research use only
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