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Human/Mouse RBP4/Retinol-Binding Protein 4 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB34761G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 423619

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RBP4/Retinol‑Binding Protein 4
Met1-Leu201
Accession # NP_035385

Specificity

Detects mouse RBP4/Retinol‑Binding Protein 4 in direct ELISA and Western Blot. In Western blots, detects human RBP4/Retinol‑Binding Protein 4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with Retinol-binding Protein 4 (RBP4), also known as Plasma Retinol-binding Protein (1‑3). A prototypic member of the lipocalin superfamily, RBP4 has a beta‑barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes, implying that RBP4, an adipocyte-derived signal, may be a biomarker and a drug target for the two diseases. The amino acid sequence of mouse RBP4 is 99%, 86%, 83% and 75% identical to that of rat, human/chimpanzee, dog and chicken.

References

  1. Zanotti, G. and R. Berni (2004) Vitamins and Hormones 69:271.
  2. Newcomer, M.E. and D.E. Ong (2000) Biochim. Biophys. Acta 1482:57.
  3. Yang, Q. et al. (2005) Nature 436:356.

Alternate Names

RetinolBinding Protein 4

Entrez Gene IDs

5950 (Human); 19662 (Mouse); 25703 (Rat)

Gene Symbol

RBP4

UniProt

Additional RBP4/Retinol-Binding Protein 4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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