Human/Mouse ROR gammat/RORC2/NR1F3 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6006P
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 600380
Product Specifications
Immunogen
Human ROR gammat peptide
Met1-Arg10
Accession # P51449
Met1-Arg10
Accession # P51449
Specificity
Detects human and mouse ROR gammat/RORC2 in direct ELISAs. In direct ELISAs, no cross-reactivity with human and mouse RORC.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse ROR gammat/RORC2/NR1F3 PE-conjugated Antibody
Detection of ROR gammat/RORC2/NR1F3 in CD4+/CD8+Mouse Thymocytes by Flow Cytometry.
CD4+/CD8+mouse thymocytes were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PE-conjugated Monoclonal Antibody (Catalog # IC6006P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of ROR gammat/RORC2/NR1F3 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/ NR1F3 PE-conjugated Monoclonal Antibody (Catalog # IC6006P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intra-cellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse ROR gammat/RORC2/NR1F3 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) and CD4+/CD8+ mouse thymocytes were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) and CD4+/CD8+ mouse thymocytes were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 1 review rated 5 using IC6006P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Long Name
Retinoic Acid-Related Orphan Receptor gamma t
Alternate Names
RORC2
Entrez Gene IDs
6097 (Human)
Gene Symbol
RORC
UniProt
Additional ROR gamma t/RORC2/NR1F3 Products
Product Documents for Human/Mouse ROR gammat/RORC2/NR1F3 PE-conjugated Antibody
Product Specific Notices for Human/Mouse ROR gammat/RORC2/NR1F3 PE-conjugated Antibody
For research use only
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